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Coding

Part:BBa_K1453300

Designed by: Binhan Hao   Group: iGEM14_SJTU-BioX-Shanghai   (2014-10-16)

FL3-TALE

Fl3tal.png

Figure The structure of FL-TAL1

This is the TALE protein with a flexible linker 3 before it. Since we cannot connect TALE by Golden Gate method designed by 2012 Freiburg, the sequence was synthesized by Genwize company. This TALE can recognize the DNA sequence TTTGGTCATGAGAT(14bp). Moreover, we use this part with our part BBa_K14530000 to make our composite part BBa_K1453305.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 526
    Illegal BsaI.rc site found at 1138


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